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E-GEOD-60447 - Stretch-dependent genes in vascular smooth muscle cells

Status
Released on 1 July 2015, last updated on 19 August 2015
Organism
Mus musculus
Samples (11)
Array (1)
Protocols (7)
Description
Vascular smooth muscle cells (VSMCs) respond to biomechanical stretch with specific changes in gene expression which govern the phenotype of these cells. The mechanotransducer zyxin is a potential candidate for regulating the expression of such genes. Using microarrays, we compared stretch-induced gene expression in wild type and zyxin-null VSMCs to define such changes in detail. Wild type (WT) and zyxin-null VSMCs were stretched at 10% cyclic elongation for 6 hours and the changes in gene expression were compared under static and stretched conditions. Up to 3 biological replicates were used for each of the 4 sample types.
Experiment type
transcription profiling by array 
Contacts
Carsten Sticht <geo@ncbi.nlm.nih.gov>, Markus Hecker, Subhajit Ghosh
Citation
Loss of the Mechanotransducer Zyxin Promotes a Synthetic Phenotype of Vascular Smooth Muscle Cells. Ghosh S, Kollar B, Nahar T, Suresh Babu S, Wojtowicz A, Sticht C, Gretz N, Wagner AH, Korff T, Hecker M.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-60447.idf.txt
Sample and data relationshipE-GEOD-60447.sdrf.txt
Raw data (1)E-GEOD-60447.raw.1.zip
Processed data (1)E-GEOD-60447.processed.1.zip
Array designA-GEOD-18615.adf.txt
Links