Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-60432 - The Assembly of miRNA-mRNA-protein Regulatory Networks Using High-throughput Expression Data (mRNA)
Released on 1 March 2015, last updated on 7 March 2015
Analyze gene expression levels in primary trophoblasts, derived from term human placenta and cultured under standard or hypoxic conditions Human placental trophoblasts were dispersed using a trypsin-deoxyribonuclease-dispase/Percoll method, plated in 6-well plates, and maintained in standard culture conditions (O2=20%). After 4 h (defined as time 0), the plates were divided to those in continued standard culture conditions, or to culture in hypoxia (O2=0%). Cells were then harvested at 6 h, 12 h, 24 h, 48 h and 72 h, and processed for mRNA arrays
transcription profiling by array
Jean-Francois Mouillet, Thomas P Conrads, Tianjiao Chu, Yoel Sadovsky