7 protocols
AccessionType
normalization data transformation protocol
RNA samples are sent to Mogene company for further processing and data generation. ID_REF = VALUE = Normalized signal intensity
array scanning protocol
RNA samples are sent to Mogene company for further processing and data generation.
hybridization protocol
RNA samples are sent to Mogene company for further processing and data generation.
labelling protocol
RNA samples are sent to Mogene company for further processing and data generation.
growth protocol
Cells are cultured in Primaria flasks (Falcon) in CMRL 1066 medium, containing 20% foetal calf serum (FCS) (Gibco, UK); L-glutamine (4mM); insulin (0.2 unit/ml); cholera toxin (10-10M) and hydrocortisone (1μg/ml), all from Sigma. Cultures were routinely maintained at 37°C in a humidified 5% CO2 incubator.
sample treatment protocol
Cells are cultured in phenol-free CMRL-1066 medium containing 10% dextran coated-charcoal (C6241, Sigma) - stripped FBS for 72h prior to stimulation with 1 nM R1881 (NLP005005MG, PerkinElmer, Waltham, MA) or vehicle for a further 16h.
nucleic acid extraction protocol
TRIzol® (Life Technologies) used for RNA extraction.