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E-GEOD-60021 - Exosomes secreted by a nematode parasite transfer small RNAs to mammalian cells and regulate genes of the innate immune system [Litomosoides sigmodontis]

Status
Released on 26 November 2014, last updated on 28 November 2014
Organism
Litomosoides sigmodontis
Samples (2)
Protocols (3)
Description
In mammalian systems, extracellular small RNAs can operate in a paracrine manner to communicate information between cells, relying on transport within vesicles. “Foreign” small RNAs derived from bacteria, plants and parasites have also been detected in mammalian body fluids, sparking interest in whether these could mediate inter-species communication. However, there is no mechanistic framework for RNA-mediated interspecies communication and the active movement of RNA via vesicles has not been shown outside of mammals. Here we demonstrate that specific microRNAs and Y RNAs are packaged into vesicles secreted by a gastrointestinal nematode, Heligmosomoides polygyrus, which naturally infects mice. Total RNA was extracted from the serum of mice infected with Litomosoides sigmodontis at 60 days post infection
Experiment type
RNA-seq of non coding RNA 
Contacts
Alasdair Ivens <al.ivens@ed.ac.uk>, Alasdair C Ivens, Amy H Buck
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-60021.idf.txt
Sample and data relationshipE-GEOD-60021.sdrf.txt
Processed data (1)E-GEOD-60021.processed.1.zip
Links