E-GEOD-59897 - Expression profile of HCT116 cells transfected with miR-204-5p

Status
Released on 30 July 2014, last updated on 2 August 2014
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (7)
Description
We have employed whole genome microarray expression profiling as a discovery platform to screen potential target genes of miR-204-5p in colorectal cancer cell HCT116. HCT116 cells were seeded in 6-cm2 tissue culture plates and transfected with the miR-204-5p mimic or negative control (NC) using Lipofectamine 2000 (Invitrogen, USA). After propagation for 48 hours, total RNA was extracted using TRIzol reagent (Invitrogen, USA). Expression profiling was performed using an Agilent human whole genome oligo microarray chip (4×44K) (Agilent, USA) Expression profiling in HCT116 cells was measured at 48 hours after transfection with miR-204-5p or negative control. Experiments were performed using the two samples without repeat experiment.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-59897.idf.txt
Sample and data relationshipE-GEOD-59897.sdrf.txt
Raw data (1)E-GEOD-59897.raw.1.zip
Processed data (1)E-GEOD-59897.processed.1.zip
Additional data (1)E-GEOD-59897.additional.1.zip
Array designA-GEOD-13497.adf.txt
Links