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E-GEOD-59725 - RNA sequencing of monocytes and microglia from mice with experimental autoimmune encephalomyelitis illustrates a changing phenotype with disease course

Status
Released on 20 October 2014, last updated on 10 December 2014
Organism
Mus musculus
Samples (15)
Protocols (4)
Description
The role of microglia and infiltrating monocytes in experimental autoimmune encephalomyelitis (EAE) pathogenesis has been controversial. To gain insight into their respective roles, we developed a method for differentiating between microglia and infiltrating monocytes in the CNS using CD44. We used this system to monitor changes in cell number, activation status, and gene expression by RNA sequencing (RNA-Seq) over the course of disease. This in vivo characterization and RNA-Seq dataset improves our understanding of myeloid cell biology in the brain under inflammatory conditions and may lead to strategies to identify therapies for inflammatory pathways active in neuroinflammatory diseases. Pooled samples from 10 mice were analyzed for both microglia and monocytes at distinct timepoints post EAE inducation. Peritoneal macrophages were isolated and analyzed for five samples, and also in a pool.
Experiment type
RNA-seq of coding RNA 
Contacts
Jonathan Daryl Hill <jon.hill@boehringer-ingelheim.com>, Dimitria Stefanopoulos, Jonathan D Hill, Kathryn W Juchem, Louise K Modis, Nuruddeen D Lewis
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-59725.idf.txt
Sample and data relationshipE-GEOD-59725.sdrf.txt
Processed data (1)E-GEOD-59725.processed.1.zip
Links