E-GEOD-5959 - Transcription profiling of livers from female C57BL/6J and B6.C3H-6T mice to study differential gene expression in the two strains
Released on 13 November 2007, last updated on 2 October 2015
Several studies have shown that bone mineral density (BMD), a clinically measurable predictor of osteoporotic fracture, is the sum of genetic and environmental influences. In addition, serum IGF-1 levels have been correlated to both BMD and fracture risk. We previously identified a Quantitative Trait Locus (QTL) for Bone Mineral Density (BMD) on mouse Chromosome (Chr) 6 that overlaps a QTL for serum IGF-1. The B6.C3H-6T (6T) congenic mouse is homozygous for C57BL/6J (B6) alleles across the genome except for a 30 cM region on Chr 6 that is homozygous for C3H/HeJ (C3H) alleles. This mouse was created to study biology behind both the BMD and the serum IGF-1 QTLs and to identify the gene(s) underlying these QTLs. Female 6T mice have lower BMD and lower serum IGF-1 levels at all ages measured. As the liver is the major source of serum IGF-1, we examined differential expression in the livers of fasted female B6 and 6T mice by microarray. Experiment Overall Design: The experimental design of this experiment was a simple two-factor experiment, with three biological replicates of each factor (in this case mouse strain, B6.C3H-6T vs. C57BL/6J).
transcription profiling by array, co-expression, strain or line
A chromosomal inversion within a Quantitative Trait Locus has a major effect on adipogenesis and osteoblastogenesis. Ackert-Bicknell CL, Salisbury JL, Horowitz M, DeMambro VE, Horton LG, Shultz KL, Lecka-Czernik B, Rosen CJ. , Europe PMC 17584978