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E-GEOD-59084 - Effects of caffeine ingestion in Drosophila melanogaster male thorax and abdomen

Status
Released on 4 July 2014, last updated on 15 July 2014
Organism
Drosophila melanogaster
Samples (6)
Array (1)
Protocols (7)
Description
To screen for xenobiotic-metabolizing-enzyme genes (XME) induced by caffeine exposure, a microarray experiment (performed with a Drosophila pangenomic array (Agilent 4 X 44K) was carried out between control and individuals exposed to caffeine (18mM ). Among 23401 genes consistantly found in the three biological replicates, 13108 showed a significant variation. 749 genes were over-expressed whereas 1518 were under-expressed. Interestingly, several genes belonging to all major classes of detoxification enzymes including XME were among the most highly induced such as CYP12d1, CYP6a8 and CYP6d5. Caffeine ingestion induced gene expression in Drosophila melanogaster male thorax and abdomen was mesured at 12h after exposure to doses of 0, 18mM of caffeine. Three independant experiments were performed at each dose using different drosophila for each experiment.
Experiment type
transcription profiling by array 
Contacts
Stéphane Fraichard <fraichar@u-bourgogne.fr>, Alexandra Coelho, Jean-Marie Heydel
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-59084.idf.txt
Sample and data relationshipE-GEOD-59084.sdrf.txt
Raw data (1)E-GEOD-59084.raw.1.zip
Processed data (1)E-GEOD-59084.processed.1.zip
Additional data (1)E-GEOD-59084.additional.1.zip
Array designA-GEOD-7300.adf.txt
Links