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E-GEOD-58854 - 3' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

Released on 8 May 2015, last updated on 22 May 2015
Drosophila melanogaster
Samples (12)
Protocols (2)
Control (+/cbtE1-UAS-cbt RNAi) or cabut RNAi flies (Tim-gal4, UAS-cbt RNAi) were starved for 16 hours and then exposed to food containing different concentrations of sucrose: 0, 25, 50 and 100 % for 18 hours. Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) For each sucrose concentration, two samples of cabut RNAi flies and one sample of control flies were sequenced.
Experiment type
RNA-seq of coding RNA 
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing. Bartok O, Teesalu M, Ashwall-Fluss R, Pandey V, Hanan M, Rovenko BM, Poukkula M, Havula E, Moussaieff A, Vodala S, Nahmias Y, Kadener S, Hietakangas V.
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-58854.idf.txt
Sample and data relationshipE-GEOD-58854.sdrf.txt
Processed data (12)Click to browse processed data
Additional data (1)