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E-GEOD-58659 - Transcript profile comparison of Zbtb20-sufficient and Zbtb20-deficient polyclonal bone marrow plasma cells

Status
Released on 19 June 2014, last updated on 29 June 2014
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (7)
Description
ZBTB20 is an adjuvant-specific factor for long-term antibody responses. This factor is critical for maintaining long-lived plasma cells in alum-adjuvanted antibody responses but is dispensable for TLR ligand-adjuvanted responses. To identify the functions of ZBTB20 in long-lived plasma cells, we performed microarray analysis on Zbtb20-sufficient and Zbtb20-deficient polyclonal bone marrow plasma cells under the assumption that ZBTB20 regulates relevant targets in all long-lived plasma cells, irrespective of their mode of formation. Chimeras were generated using Zbtb20-sufficient (WT) or Zbtb20-deficient (TRAP) E14.5 fetal livers. 3-4 months after reconstitution, donor bone marrow B220low/-CD138+ cells (4 replicates per genotype) were purified via FACS for microarray. In total, 8 samples, 4 for each genotype, were included in this study.
Experiment type
transcription profiling by array 
Contacts
Yinan Wang <u0203551@gmail.com>, Deepta Bhattacharya
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-58659.idf.txt
Sample and data relationshipE-GEOD-58659.sdrf.txt
Raw data (1)E-GEOD-58659.raw.1.zip
Processed data (1)E-GEOD-58659.processed.1.zip
Array designA-AFFY-130.adf.txt
Links