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E-GEOD-58333 - Genome-wide profiling of Zfp335 binding sites in thymocytes

Status
Released on 17 October 2014, last updated on 10 December 2014
Organism
Mus musculus
Samples (6)
Protocols (4)
Description
The generation of naïve T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Mutant blt/blt mice exhibit a naïve T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. Zfp335 binds to promoter regions via a consensus motif, and its target genes are enriched in categories related to protein metabolism, mitochondrial function and transcriptional regulation. Restoring the expression of one target, Ankle2, partially rescues T cell maturation. Our findings identify Zfp335 as a transcription factor and essential regulator of late-stage intrathymic and post-thymic T cell maturation. 4 Zfp335 ChIP-seq samples and 2 input samples with WT vs. blt/blt thymocytes.
Experiment type
ChIP-seq 
Contacts
Brenda Yuyuan Han <yuyuanhan@gmail.com>, Belinda Whittle, Brenda Y Han, Christopher C Goodnow, Chuan S Foo, Craig N Jenne, Jason G Cyster, Robert M Horton, Shuang Wu, Susan R Watson
Citation
Zinc finger protein Zfp335 is required for the formation of the na�ve T cell compartment. Han BY, Wu S, Foo CS, Horton RM, Jenne CN, Watson SR, Whittle B, Goodnow CC, Cyster JG. , PMID:25343476
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-58333.idf.txt
Sample and data relationshipE-GEOD-58333.sdrf.txt
Processed data (2)E-GEOD-58333.processed.1.zip, E-GEOD-58333.processed.2.zip
Links