E-GEOD-5736 - Transcription profiling by array of Arabidopsis young and old siliques

Released on 14 June 2008, last updated on 17 January 2012
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (2)
The aim of the experiment is to compare and contrast the profiles of gene expression during silique senescence and to identify the molecular events that regulate the timing of the process. Silique occurs over a highly predictable time frame and as a consequence it may be easier to identify the molecular events that are critical for the process to take place than in systems such as leaf senescence. The material will be collected from 20 Arabidopsis thaliana ecotype Columbia plants (NASC order number N1093) grown under controlled conditions specified under NASC protocol: Baseline sample preparation. Two pods will be collected from each plant and pooled from 20 plants to reduce sampling variability. It is intended that 3 replicates of the experiment will be submitted. The RNA will be extracted from mature green silique tissues of 10 days after anthesis and senescing pod tissues of 20 days after anthesis using lithium chloride extraction protocol and cleaned with Qiagen RNAeasy column. Experimenter name = Thomas Yang; Experimenter phone = 01159516378; Experimenter institute = University of Nottingham; Experimenter address = Division of Plant Sciences; Experimenter address = University of Nottingham; Experimenter address = Sutton Bonington Campus; Experimenter address = Loughborough; Experimenter address = Leicestershire; Experimenter zip/postal_code = LE12 5RD; Experimenter country = UK Experiment Overall Design: 6 samples were used in this experiment
Experiment types
transcription profiling by array, unknown experiment type
Investigation descriptionE-GEOD-5736.idf.txt
Sample and data relationshipE-GEOD-5736.sdrf.txt
Raw data (1)E-GEOD-5736.raw.1.zip
Processed data (1)E-GEOD-5736.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-5736.eSet.r