E-GEOD-57356 - Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts

Released on 7 May 2014, last updated on 12 May 2014
Homo sapiens
Samples (16)
Array (1)
Protocols (7)
Exposure of eEC and eSF to SP in vitro increases expression of genes and secreted proteins associated with cellular migration, proliferation, viability and inhibition of cell death. This is a cross sectional study involving control samples versus treatment. SP (pooled from twenty donors) was first tested for dose- and time-dependent cytotoxic effects on eEC and eSF (n = 4). As exposure of eEC or eSF to 1% SP for 6 h proved to be non-toxic, a second set of eEC/eSF samples (n = 4) was treated under these conditions for transcriptome, protein and functional analysis. With a third set of samples (n = 3), we further compared the transcriptional response of the cells to SP versus fresh semen.
Experiment type
transcription profiling by array 
Linda Giudice <giudice@obgyn.ucsf.edu>, Brittni A Johnson, David W Erikson, Fatima Barragan, Joseph C Chen, Juan C Irwin, Linda C Giudice, Nadia R Roan, Nargis Kohgadai, Simon Chu, Terhi T Piltonen, Warner C Greene
Investigation descriptionE-GEOD-57356.idf.txt
Sample and data relationshipE-GEOD-57356.sdrf.txt
Raw data (1)E-GEOD-57356.raw.1.zip
Processed data (1)E-GEOD-57356.processed.1.zip
Array designA-AFFY-141.adf.txt