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E-GEOD-57263 - Comparison of gene expression of WT and PerM mutant M. tuberculosis H37Rv grown in high and reduced magnesium

Status
Released on 5 January 2015, last updated on 10 January 2015
Organism
Mycobacterium tuberculosis
Samples (12)
Array (1)
Protocols (6)
Description
M. tuberculosis membrane protein PerM is required for persistence in chronic mouse infection. In vitro, the perM mutant requires increased magnesium for replication and survival, with elongation and lysis in low-magnesium media. To gain insight into the function of PerM, we compared the transcriptomes of wt and perM mutant Mtb grown in high (2000 μM) and reduced (250 μM) Mg2+ in three independent experiments. Transcriptome analysis of the perM mutant grown in reduced magnesium revealed upregulation of cell division and cell wall biosynthesis genes. Bacteria were grown for 5 days in Sauton’s media containing 250 or 2000 μM MgCl2 and 0.05% Tween 80. Three independent experiments were performed.
Experiment type
transcription profiling by array 
Contacts
Nichole Goodsmith, Sabine Ehrt
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-57263.idf.txt
Sample and data relationshipE-GEOD-57263.sdrf.txt
Raw data (1)E-GEOD-57263.raw.1.zip
Processed data (1)E-GEOD-57263.processed.1.zip
Additional data (1)E-GEOD-57263.additional.1.zip
Array designA-GEOD-16177.adf.txt
Links