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E-GEOD-56923 - Comparison of Treg and Teff cells in healthy and COPD smokers

Status
Released on 19 April 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (6)
Description
Rationale: COPD is characterized by an abnormal regulatory T cell (Treg) response and increases in Th1 and Th17 cell responses. It is unclear if dysregulation of miRNAs within Treg alters the adaptive immunophenotype in COPD. Objectives: To compare the miRNA profile of COPD Treg cells with that of healthy controls and to explore the function of differentially expressed miRNAs. Methods: Treg cells (CD4+CD25+CD49-CD127-) and T effector (CD4+CD25-) cells were obtained from the peripheral blood of 4 nonsmokers, 4 healthy current smokers, and 4 COPD current smokers for analysis of miRNA expression, matching them for age and sex. We assessed expression initially by microarray analysis on the Illumina miRNA platform then conducted real time RT-PCR validation of the microarray results. 24 samples were analyzed. 8 from each patient group of healthy Normal, Healthy Smoker, and COPD Smoker.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-56923.idf.txt
Sample and data relationshipE-GEOD-56923.sdrf.txt
Processed data (1)E-GEOD-56923.processed.1.zip
Additional data (1)E-GEOD-56923.additional.1.zip
Array designA-GEOD-8179.adf.txt
Links