E-GEOD-56514 - Gene expression changes in CD4+ thymocytes upon removal of Cullin3
Released on 5 April 2014, last updated on 3 June 2014
We report gene expression changes in Cul3 deficient thymic CD4+ T cells We used microarrays to detail the global programme of gene expression changes upon removal of Cul3 during thymic development CD25-, CD1dPBS57-, gdTCR - CD4+ Single positive cells from thymocytes of CD4Cre-Cul3fl/fl (LM) and CD4 Cre+Cul3fl/fl (KO) were sort purified and total RNA was extracted using Trizol and analysed by microarray.
transcription profiling by array
Rebecca Mathew <email@example.com>, Aaron R Dinner, Ai-ping Mao, Albert Bendelac, Alexander L Dent, Andrew H Chiang, Benjamin D McDonald, Clara Bertozzi-Villa, Jeffrey D Singer, Jeffrey J Bunker, Kristin Hollister, Michael G Constantinides, Seth T Scanlon