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E-GEOD-56272 - The expression of small RNAs after prg-1 mutation

Status
Released on 9 June 2014, last updated on 8 September 2014
Organism
Caenorhabditis elegans
Samples (6)
Protocols (3)
Description
Piwi-related Argonaute proteins play important roles in maintaining germline integrity and fertility and have been linked to a class of germline-enriched small RNAs termed piRNAs. Caenorhabditis elegans encodes two Piwi family proteins called PRG-1 and PRG-2, and PRG-1 interacts with the C. elegans piRNAs (21U-RNAs). Previous studies found that the prg-1 mutation causes a marked reduction in the expression of 21U-RNAs, temperature-sensitive defects in fertility and other phenotypic defects.To systematically demonstrate the function of PRG-1 on regulating small RNAs and their targets. We use recent advances in high-throughput sequencing technology to show that expression of non-coding small RNAs in six stages(embryo,L1,L2,L3,L4,young audlt) and mRNAs in four stages (L1,L2,L3,L4) after prg-1 mutation. prg-1 mutation can not only lead to a decrease in the expression of 21U-RNAs, but also cause 35~40% of miRNAs to be significantly down-regulated; approximately 3% (6.00% in L4) of protein-coding genes are differentially expressed after mutating prg-1, and 60~70% of these substantially changed protein-coding genes are up-regulated. Examination of small RNA expression in six different developmental stages (embryo, L1, L2, L3, L4, young adult) and mRNA expression in four stages (L1,L2,L3,L4) of C. elegans prg-1 mutant (wm161) .
Experiment type
RNA-seq of non coding RNA 
Contacts
Jiajia Wang <wangjiajia_816@126.com>, Dong-Ya Cui, Jia-Jia Wang, Peng Zhang, Shunmin He, Tengfei Xiao
Citation
The influences of PRG-1 on the expression of small RNAs and mRNAs. Wang JJ, Cui DY, Xiao T, Sun X, Zhang P, Chen R, He S, Huang DW. , PMID:24884413
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-56272.idf.txt
Sample and data relationshipE-GEOD-56272.sdrf.txt
Processed data (1)E-GEOD-56272.processed.1.zip
Links