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E-GEOD-56101 - Heterochromatin-associated interactions of Drosophila HP1 with dADD1, HIPP1, and repetitive RNAs

Status
Released on 3 July 2014, last updated on 6 September 2014
Organism
Drosophila melanogaster
Samples (27)
Protocols (5)
Description
We report chromatin-associated protein and RNA interactions of HP1a, indentified by BioTAP-XL mass spectrometry and sequencing. We identify an extensive list of both known and novel HP1a-interacting proteins from Drosophila S2 cells and from whole organisms across embryonic, larval and adult stages. BioTAP-XL protocol was used to identify HP1a interacting proteins through mass-spectrometry analysis. ChIP-seq-like pulldown/input samples were generated using BioTAP-XL to validate genomic distribution of the HP1a-BioTAP contructs, and the newly identified interacting proteins (CG8290 and CG3680); To examine the RNAs associated with the HP1a complexes, the RNA fraction of the BioTAP-XL pulldowns was analyzed using Illumina-based RNA-seq protocols, as well as Helicos direct RNA sequencing.
Experiment types
ChIP-seq, RNA-seq of coding RNA 
Contacts
Peter Kharchenko <geo@ncbi.nlm.nih.gov>, Art A Alekseyenko, Mitzi I Kuroda, Peter V Kharchenko
Citation
Heterochromatin-associated interactions of Drosophila HP1a with dADD1, HIPP1, and repetitive RNAs. Alekseyenko AA, Gorchakov AA, Zee BM, Fuchs SM, Kharchenko PV, Kuroda MI. , PMID:24990964
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-56101.idf.txt
Sample and data relationshipE-GEOD-56101.sdrf.txt
Processed data (15)Click to browse processed data
Links