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E-GEOD-56084 - Transcription profiling by array of human MDA-MB-231 breast cancer cells with ATAD3A stable knockdown against no-knockdown controls

Status
Released on 1 March 2015, last updated on 30 April 2015
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
From our previous data, we found that loss of ATAD3A gene expression in breast cancer cells results in loss of cell motility in vitro and metastasis in vivo. To obtain a better understanding of oncogenic pathway of ATAD3A, we have established the stable ATAD3A knockdown MDA-MB-231 cells using lentiviral strategy. We used the whole genome microarrays to detail the global programme of gene expression after depleting of ATAD3A and identified distinct classes of up or down-regulated metastmir associated with breast cancer cells migration Total RNA was extracted from ATAD3A stable knockdown cells (shATAD3A) and the control cells (shGFP). The labeled RNA was hybridized on U133 plus 2.0 Array. To identify altered gene expression patterns with or without ATAD3A expression, we compared average mRNA expression levels between the ATAD3A knockdown and control MDA-MB-231 cells.
Experiment type
transcription profiling by array 
Contacts
yong teng <yteng@gru.edu>, J K Cowell, Yong Teng
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-56084.idf.txt
Sample and data relationshipE-GEOD-56084.sdrf.txt
Raw data (1)E-GEOD-56084.raw.1.zip
Processed data (1)E-GEOD-56084.processed.1.zip
Array designA-AFFY-44.adf.txt
Links