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E-GEOD-55844 - Gene expression in FIH-1 silenced human nucleus pulposus cells

Status
Released on 27 May 2014, last updated on 31 May 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
To evaluate the effect of Hypoxia-inducible factor 1-alpha inhibitor (HIF-1) in nucleus pulposus (NP) cells, human NP cells were lentivirally transduced with either control or FIH-1 targeted shRNA. Gene expression changes between samples from control and FIH-1 silenced cells were evaluated using a microarray. Primary NP cells were isolated from human discs and grown in culture. Cells were transduced with either control or FIH-1 targeting shRNA constructs. Silencing of FIH-1 was confirmed for each set using qRT-PCR and Western blot. Total RNA from NP cells was used for RNA extraction and hybridization on the Affymetrix microarray. Control samples: C1, C2, and C3 and FIH-1-silenced samples: E1, E2, and E3.
Experiment type
transcription profiling by array 
Contacts
Makarand Risbud <makarand.risbud@jefferson.edu>, D Markova, I Shapiro, K Chiba, M Risbud, Y Hirose, Y Toyama, Z Johnson, Z Schoepflin
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55844.idf.txt
Sample and data relationshipE-GEOD-55844.sdrf.txt
Raw data (1)E-GEOD-55844.raw.1.zip
Processed data (1)E-GEOD-55844.processed.1.zip
Additional data (1)E-GEOD-55844.additional.1.zip
Array designA-AFFY-141.adf.txt
Links