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E-GEOD-55844 - Gene expression in FIH-1 silenced human nucleus pulposus cells

Released on 27 May 2014, last updated on 31 May 2014
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
To evaluate the effect of Hypoxia-inducible factor 1-alpha inhibitor (HIF-1) in nucleus pulposus (NP) cells, human NP cells were lentivirally transduced with either control or FIH-1 targeted shRNA. Gene expression changes between samples from control and FIH-1 silenced cells were evaluated using a microarray. Primary NP cells were isolated from human discs and grown in culture. Cells were transduced with either control or FIH-1 targeting shRNA constructs. Silencing of FIH-1 was confirmed for each set using qRT-PCR and Western blot. Total RNA from NP cells was used for RNA extraction and hybridization on the Affymetrix microarray. Control samples: C1, C2, and C3 and FIH-1-silenced samples: E1, E2, and E3.
Experiment type
transcription profiling by array 
Makarand Risbud <>, D Markova, I Shapiro, K Chiba, M Risbud, Y Hirose, Y Toyama, Z Johnson, Z Schoepflin
Investigation descriptionE-GEOD-55844.idf.txt
Sample and data relationshipE-GEOD-55844.sdrf.txt
Raw data (1)
Processed data (1)
Additional data (1)
Array designA-AFFY-141.adf.txt