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E-GEOD-55786 - Gene expression of T47D-MTVL human breast cancer cells upon H1.4 knock-down or overexpression

Status
Released on 16 May 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (16)
Array (1)
Protocols (7)
Description
Gene expression of T47D-MTVL human breast cancer cells expressing Dox-inducible shRNAs against histone H1.4 (sh120) or multiple H1 variants (sh225), or overexpressing WT or K26A mutant HA-tagged H1.4. T47D-MTVL, breast cancer cell line carrying one stably integrated copy of luciferase reporter gene driven by the MMTV promoter, is stably infected with an inducible system for the expression of shRNAs.Cells stably express RedFP and KRAB repressor fused to Tet regulator.Upon Dox treatment, cells express RedFP and the cloned shRNA. Stable breast cancer-derived cell lines expressing an shRNA against one of each of the histone H1 isoforms in response to doxycycline (Dox) were grown for six days in the presence or absence of Dox, in duplicate, and RNA extracted for microarray hybridization. Cell lines used: inducible shRNA against H1.4 or multiple H1 variants, and random shRNA-expression vector. Stable breast cancer-derived cell lines expressing the histone H1.4 isoform, WT or K26A, HA-tagged, in duplicate, and RNA extracted for microarray hybridization. Cell lines used: overexpressing H1.4 WT or K26A mutant.
Experiment type
transcription profiling by array 
Contacts
Albert Jordan, Jean-Michel Terme, Lluís Millán-Ariño
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55786.idf.txt
Sample and data relationshipE-GEOD-55786.sdrf.txt
Processed data (1)E-GEOD-55786.processed.1.zip
Additional data (1)E-GEOD-55786.additional.1.zip
Array designA-GEOD-18405.adf.txt
Links