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E-GEOD-55670 - High intake of dietary sugar enhances bisphenol A (BPA) disruption and reveals ribosome-mediated pathways of toxicity: Chronic Exposure

Status
Released on 4 June 2014, last updated on 6 June 2014
Organism
Drosophila melanogaster
Samples (32)
Array (1)
Protocols (5)
Description
Fly strain y[1]; bw[1]; e[4]; ci[1] ey[R] was obtained from the Bloomington Stock Center. Flies were reared in incubators at 25°C, 65% of relative humidity, and photoperiod of 12 hours. For gene expression analyses flies were reared in media containing BPA, high sugar, and BPA + high sugar (chronic exposure). After development, newly emerged adult male flies were collected, aged for 48 hours, and flash frozen in liquid nitrogen. We collected four replicas of each sample and stored tehm at -80°C. Total RNA was extracted from whole flies using TRIzol (Life Technologies). The synthesis of cDNA and its labeling with fluorescent dyes (Cy3 and Cy5) as well as hybridization reactions were carried out using 3DNA protocols and reagents (Genisphere). Slides were scanned using Axon 400B scanner (Axon Instruments) and GenePix Pro 6.0 software. Foreground fluorescence of dye intensities was normalized by the Loess method in Bioconductor / Limma. Dye "swaps," loop design.
Experiment type
transcription profiling by array 
Contacts
Alan Branco <geo@ncbi.nlm.nih.gov>, Alan T Branco, Bernardo Lemos
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55670.idf.txt
Sample and data relationshipE-GEOD-55670.sdrf.txt
Raw data (1)E-GEOD-55670.raw.1.zip
Processed data (1)E-GEOD-55670.processed.1.zip
Array designA-GEOD-6056.adf.txt
Links