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E-GEOD-55239 - Acinetobacter oleivorans DR1 : Wild-type cell harboring plasmid (pAST2) in nutrient media

Released on 22 February 2014, last updated on 6 May 2014
Acinetobacter oleivorans DR1
Samples (1)
Protocols (2)
Transcriptional profiling of A. oleivorans DR1 cells harboring pAST2. Plasmid pAST2 is a tetracycline-resistance plasmid which was isolated from activated sludge (Hong et al., 2014). The complete plasmid sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank under accession number KC734561 [PMID: 24337108]. Acquisition of TC resistance through plasmid uptake is related to loss of biological fitness and affected host gene expression in oil-degrading soil bacterium, Acinetobacter oleivorans DR1. To identify genes in A. oleivorans DR1 harboring pAST2, the cells were grown to exponential phase (OD600 ~0.4) and total RNA was extracted using an RNeasy Mini kit (Qiagen, USA) following the manufacturer's instructions.
Experiment type
RNA-seq of coding RNA 
Hyerim Hong
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-55239.idf.txt
Sample and data relationshipE-GEOD-55239.sdrf.txt
Processed data (1)