E-GEOD-55227 - Gene expression analysis of Cbfb-deficient LSK and GMP

Status
Released on 5 April 2014, last updated on 8 April 2014
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (7)
Description
Runx/Cbfb heterodimers play important roles in the development of hematopoietic cells in mouse embryos and adults. In order to identify genes that are regulated by Runx/Cbfb, we purified Lin– c-kit+ Sca1+ (LSK) cells and Lin– c-kit+ Sca1– CD16/32+ (GMP) cells from Vav1-iCre x Cbfb(F/F) and Vav1-iCre x Cbfb(F/+) mice and profiled gene expression using microarray. 200,000 LSK and GMP cells were purified separately from two 7 week old Vav1-iCre x Cbfb(F/F) mice and two Vav1-iCre x Cbfb(F/+) mice by cell sorting. The purity was higher than 98%. Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel), amplified using a PicoSL RNA amplification kit (Nugen) and biotinylated with Encore biotin module (Nugen). Labeled RNA was hybridized to Mouse Gene 1.0ST microarrays (Affymetrix) according to the manufacturer’s instruction.
Experiment type
transcription profiling by array 
Contacts
Chun Chou, Takeshi Egawa
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55227.idf.txt
Sample and data relationshipE-GEOD-55227.sdrf.txt
Raw data (1)E-GEOD-55227.raw.1.zip
Processed data (1)E-GEOD-55227.processed.1.zip
Array designA-AFFY-130.adf.txt
Links