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E-GEOD-55151 - Differential expression of miRNAs and their target genes in senescing leaves and siliques: insights from massive sequencing of small RNAs and cleaved target RNAs

Status
Released on 8 September 2014, last updated on 10 December 2014
Organism
Arabidopsis thaliana
Samples (14)
Protocols (3)
Description
To identify senescence-regulation of miRNAs in Arabidopsis thaliana, eight small RNA libraries were constructed and sequenced at four different stages of development and senescence from both leaves and siliques. Parallel Analysis of RNA Ends (PARE) libraries were also constructed and sequenced to enable the large-scale examination of miRNA-guided cleavage products. Genome-wide small RNA profiling was done by Illumina TruSeq sample preparation followed by high-throughput sequencing with Illumina HiSeq 2000 platform. 
Experiment types
other, RNA-seq of non coding RNA 
Contacts
Pamela J Green, Shawn R Thatcher
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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