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E-GEOD-55054 - Astrocyte-encoded positional cues maintain sensorimotor circuit integrity

Status
Released on 2 May 2014, last updated on 12 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
Astrocytes, the most abundant cells in the central nervous system, promote synapse formation and help refine neural connectivity. Although they are allocated to spatially distinct regional domains during development, it is unknown whether region-restricted astrocytes are functionally heterogeneous. Here we show that postnatal spinal cord astrocytes express several region-specific genes, and that ventral astrocyte-encoded Semaphorin3a (Sema3a) is required for proper motor neuron and sensory neuron circuit organization. Loss of astrocyte-encoded Sema3a led to dysregulated α−motor neuron axon initial segment orientation, markedly abnormal synaptic inputs, and selective death of α−but not of adjacent γ−motor neurons. Additionally, a subset of TrkA+ sensory afferents projected to ectopic ventral positions. These findings demonstrate that stable maintenance of a positional cue by developing astrocytes influences multiple aspects of sensorimotor circuit formation. More generally, they suggest that regional astrocyte heterogeneity may help to coordinate postnatal neural circuit refinement. 12 total samples consisting of three biological replicates each of flow sorted postnatal day 7 dorsal spinal cord astrocytes, ventral spinal cord astrocytes, dorsal SC non astrocytes, and ventral SC non astrocytes
Experiment type
transcription profiling by array 
Contacts
David Rowitch <geo@ncbi.nlm.nih.gov>, A V Molofsky, D H Rowitch
Citation
Astrocyte-encoded positional cues maintain sensorimotor circuit integrity. Molofsky AV, Kelley KW, Tsai HH, Redmond SA, Chang SM, Madireddy L, Chan JR, Baranzini SE, Ullian EM, Rowitch DH. , PMID:24776795
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55054.idf.txt
Sample and data relationshipE-GEOD-55054.sdrf.txt
Raw data (1)E-GEOD-55054.raw.1.zip
Processed data (1)E-GEOD-55054.processed.1.zip
Additional data (1)E-GEOD-55054.additional.1.zip
Array designA-AFFY-130.adf.txt
Links