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E-GEOD-54491 - Identification of stable markers of the EMT:MET process

Status
Released on 30 January 2014, last updated on 18 February 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) facilitate breast cancer (BC) metastasis, however stable molecular changes that result as a consequence of these processes remain poorly defined. Therefore, we sought to identify molecular markers that could distinguish tumor cells that had completed the EMT:MET cycle in the hopes of identifying and targeting unique aspects of metastatic tumor outgrowth.Therefore, normal murine mammary gland (NMumG) cells transformed by overexpression of EGFR (NME) cells were cultured in the presence of TGF-beta1 (5 ng/ml) for 4 weeks, at which point TGF-beta1 supplementation was discontinued and the cells were allowed to recover for an additional 4 weeks (Post-TGF-Rec). Total RNA was prepared from unstimulated cells (Pre-TGF) of similar passage and compared by microarray analysis. The two groups were analyzed in triplicate, three Pre-TGF samples and three Post-TGF-Rec samples.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-54491.idf.txt
Sample and data relationshipE-GEOD-54491.sdrf.txt
Raw data (1)E-GEOD-54491.raw.1.zip
Processed data (1)E-GEOD-54491.processed.1.zip
Additional data (1)E-GEOD-54491.additional.1.zip
Array designA-AFFY-130.adf.txt
Links