Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-53965 - Transcription profiling by array of human HCT116 cells treated with Nutlin-3 (an inhibitor of mdm2 and p53 interaction) or DMSO control to study p53-regulated transcription
Released on 15 May 2014, last updated on 1 May 2015
HCT116 microarray done 12 hours after treatment with DMSO (control) or Nutlin Total RNA from HCT116 cells was harvested with an RNeasy kit (Qiagen) and analyzed on Affymetrix HuGene 1.0 ST arrays following the manufacturer’s instructions. Microarray data were processed using Partek Genomics Suite 6.6. Anova was used to call differentially expressed genes for which any isoform showed a fold change > |+/-1.5| with FDR <0.05. There were 362 genes called as upregulated and 367 genes as downregulated.
transcription profiling by array
Mary Ann Allen <firstname.lastname@example.org>, Joaquin Espinosa, Mary A Allen, Robin D Dowell