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E-GEOD-53899 - Effect of neurotrophin receptor p75 (p75NTR) over-expression on the miRNAome of human umbilical vein endothelial cells

Status
Released on 11 January 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (6)
Description
MicroRNAs (miRNAs) are small non-protein-coding RNAs that are incorporated into the RNA-induced silencing complex (RISC) and inhibit gene expression by regulating the stability and/or the translational efficiency of target mRNAs. p75NTR, which is scarcely present in healthy endothelial cells (ECs), becomes strongly expressed by capillary ECs after induction of peripheral ischemia in type-1 diabetic mice. p75NTR expression promotes endothelial cells apoptosis and inhibits angiogenesis. In order to identify miRNAs sub-sequentely modulated by p75NTR, miRNA expression profiles of human umbilical vein endothelial cells (HUVEC) over-expressing p75NTR were generated, allowing the identification of miRNAs modulated upon p75NTR up-regulation. HUVEC over-expressing p75NTR or Null (empty vector) were generated by adenoviral infection. miRNA expression profiles were then measured and miRNAs modulated upon p75NTR up-regulation were identified.
Experiment type
transcription profiling by array 
Contacts
Andrea Caporali <a.caporali@ed.ac.uk>, A Caporali, C Emanueli, C Voellenkle, F Martelli
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-53899.idf.txt
Sample and data relationshipE-GEOD-53899.sdrf.txt
Raw data (1)E-GEOD-53899.raw.1.zip
Additional data (1)E-GEOD-53899.additional.1.zip
Array designA-MEXP-1469.adf.txt
Links