E-GEOD-5379 - Transcription profiling of mouse C3H10T1/2 cells treated with Glis3 identifies its role in osteoblast differentiation and adipocyte differentiation
Submitted on 24 July 2006, released on 13 June 2008, last updated on 4 May 2014
The expression of Glis3 in C3H10T1/2 cells promotes osteoblastic differentiation as indicated by the the induction of increase in alkaline phosphatase activity, an early marker of osteoblast differentiation, and increased expression of osteopontin, a late marker of osteogenesis. Glis3 acts synergistically with bone morphogenic protein 2 (BMP-2). In contrast, expression of Glis3 inhibits the induction of adipocyte differentiation. Microarray analysis identified the fibroblast growth factor 18 (FGF18) as one of the genes induced by Glis3 in C3H10T1/2 cells directly. Experiment Overall Design: Total RNA was extracted from C3H10T1/2 cells infected with empty vector or Glis3 using Qiagen Rneasy Mini kit. Each RNA sample was amplified and converted to fluorescently labeled cRNA, either with cyanine 3 (Cy3) or cyanine 5 (Cy5), using the Agilent Fluorescent Linear Amplification Kit. The fluorescently labeled cRNAs were mixed and hybridized simultaneously to Agilent mouse oligo arrays. The sample pair was hybridized to two arrays, employing a fluor reversal. Chips were scanned with an Agilent dual-laser scanner. Gene expression data were generated using the Agilent feature extraction software (v7.5), with defaults for all parameters.
transcription profiling by array, unknown experiment type
Krüppel-like zinc finger protein Glis3 promotes osteoblast differentiation by regulating FGF18 expression. Ju Youn Beak, Hong Soon Kang, Yong-Sik Kim, Anton M Jetten. J Bone Miner Res 22(8):1234-44 (2007)