E-GEOD-5379 - Transcription profiling of mouse C3H10T1/2 cells treated with Glis3 identifies its role in osteoblast differentiation and adipocyte differentiation

Status
Submitted on 24 July 2006, released on 13 June 2008, last updated on 4 May 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (1)
Description
The expression of Glis3 in C3H10T1/2 cells promotes osteoblastic differentiation as indicated by the the induction of increase in alkaline phosphatase activity, an early marker of osteoblast differentiation, and increased expression of osteopontin, a late marker of osteogenesis. Glis3 acts synergistically with bone morphogenic protein 2 (BMP-2). In contrast, expression of Glis3 inhibits the induction of adipocyte differentiation. Microarray analysis identified the fibroblast growth factor 18 (FGF18) as one of the genes induced by Glis3 in C3H10T1/2 cells directly. Experiment Overall Design: Total RNA was extracted from C3H10T1/2 cells infected with empty vector or Glis3 using Qiagen Rneasy Mini kit. Each RNA sample was amplified and converted to fluorescently labeled cRNA, either with cyanine 3 (Cy3) or cyanine 5 (Cy5), using the Agilent Fluorescent Linear Amplification Kit. The fluorescently labeled cRNAs were mixed and hybridized simultaneously to Agilent mouse oligo arrays. The sample pair was hybridized to two arrays, employing a fluor reversal. Chips were scanned with an Agilent dual-laser scanner. Gene expression data were generated using the Agilent feature extraction software (v7.5), with defaults for all parameters.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Krüppel-like zinc finger protein Glis3 promotes osteoblast differentiation by regulating FGF18 expression. Ju Youn Beak, Hong Soon Kang, Yong-Sik Kim, Anton M Jetten. J Bone Miner Res 22(8):1234-44 (2007)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5379.idf.txt
Sample and data relationshipE-GEOD-5379.sdrf.txt
Processed data (1)E-GEOD-5379.processed.1.zip
Array designA-AGIL-8.adf.txt
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