E-GEOD-5348 - Transcription profiling of mouse model of Wilson disease reveals specific changes of liver transcriptome in the early stages of copper accumulation

Status
Submitted on 19 July 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (4)
Description
Wilson disease (WD) is a severe metabolic disorder caused by genetic inactivation of copper-transporting ATPase ATP7B. In WD, copper accumulates in several tissues, particularly in the liver, inducing marked time-dependent pathological changes. To identify initial events in the copper-dependent development of liver pathology we utilized the Atp7b-/- mice, an animal model for WD. Analysis of mRNA from livers of control and Atp7b-/- 6 weeks-old mice using oligonucleotide arrays revealed specific changes of the transcriptome at this stage of copper accumulation. Few messages (29 up-regulated and 46 down-regulated) change their abundance more than 2-fold pointing to the specific effect of copper on gene expression/mRNA stability. The gene ontology analysis revealed copper effects on distinct metabolic pathways. Experiment Overall Design: RNA isolation for microarrays: Immediately after removal, what size [mg] the liver pieces for RNA isolation were frozen in liquid nitrogen and stored till further use. The total RNA was isolated from frozen mouse livers using TRIZOL reagent (Invitrogen) according to manufacturer’s recommendations followed by a subsequent sample
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
High copper selectively alters lipid metabolism and cell cycle machinery in the mouse model of Wilson disease. Dominik Huster, Tina D Purnat, Jason L Burkhead, Martina Ralle, Oliver Fiehn, Franziska Stuckert, N Erik Olson, Daniel Teupser, Svetlana Lutsenko. J Biol Chem 282(11):8343-55 (2007)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5348.idf.txt
Sample and data relationshipE-GEOD-5348.sdrf.txt
Raw data (1)E-GEOD-5348.raw.1.zip
Processed data (1)E-GEOD-5348.processed.1.zip
Array designA-AFFY-36.adf.txt
R ExpressionSetE-GEOD-5348.eSet.r
Links