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E-GEOD-53354 - Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation

Status
Released on 22 January 2014, last updated on 30 May 2014
Organism
Gallus gallus
Samples (2)
Protocols (2)
Description
Splicing factor SRSF10 is known to function as a sequence-specific splicing activator. Here, we used RNA-seq coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. We found that SRSF10 promoted both exon inclusion and exclusion. Functionally, many of SRSF10-verified alternative exons are linked to pathways of stress and apoptosis. Importantly, reconstituted SRSF10 in knockout cells recovered wild-type splicing patterns and considerably rescued the stress-related defects. Together, our results provide mechanistic insight into SRSF10-regulated alternative splicing events in vivo and demonstrate that SRSF10 plays a crucial role in cell survival under stress conditions. RNA-seq for wide type (WT) and SRSF10-deficient (KO) chicken DT40 cells
Experiment type
RNA-seq of coding RNA 
Contacts
Wenwu Wu, Ying Feng
Citation
Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation. Zhou X, Wu W, Li H, Cheng Y, Wei N, Zong J, Feng X, Xie Z, Chen D, Manley JL, Wang H, Feng Y. , PMID:24442672
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-53354.idf.txt
Sample and data relationshipE-GEOD-53354.sdrf.txt
Processed data (2)E-GEOD-53354.BAM.SRR1050759.bam, E-GEOD-53354.BAM.SRR1050760.bam
Additional data (1)E-GEOD-53354.additional.1.zip
Links