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E-GEOD-53278 - Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

Status
Released on 18 July 2014, last updated on 6 September 2014
Organism
Homo sapiens
Samples (5)
Protocols (4)
Description
We identified miRNAs differentially regulated upon Salmonella infection by comparative deep-sequencing analysis of cDNA libraries prepared from the small RNA population (10–29 nt) of HeLa cells infected with Salmonella (20 hpi) and mock-treated cells. Considering that at a MOI of 25 Salmonella is internalized in only 10-15% of the HeLa cells, we separated the fraction of cells which had internalized Salmonella (Salmonella+) from the bystander fraction (Salmonella-) by fluorescence-activated cell sorting (FACS), and extended the analysis of miRNA changes to these samples. Interestingly, we observed that Salmonella infection induces a significant decrease in the expression of all the detected members of the miR-15 family miRNA profiles of HeLa cells infected with Salmonella Typhimurium were generated by deep sequencing, using Illumina HiSeq2000.
Experiment type
RNA-seq of non coding RNA 
Contacts
Konrad U. Förstner <konrad.foerstner@uni-wuerzburg.de>, Ana Eulalio, Claire Maudet, Konrad U Förstner, Malvika Sharan, Mauro Giacca, Miguel Mano, Ushasree Sunkavalli
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-53278.idf.txt
Sample and data relationshipE-GEOD-53278.sdrf.txt
Processed data (1)E-GEOD-53278.processed.1.zip
Links