E-GEOD-5313 - Transcription profiling of mouse carotid bodies from A/J vs.DBA/2J
Submitted on 14 July 2006, released on 13 June 2008, last updated on 27 March 2012
We hypothesize that gene expression in the CBs of these two strains of mice are divergent thus contributing to the disparity in the phenotypes. More specifically, (1) genes related to CB function are expressed less in the A/J mice compared to DBA/2J mice; (2) gene expression levels of morphogenic and trophic factors of the CB maybe significantly lower in the A/J mice than the DBA/2J mice. In order to test these hypotheses, we utilized microarray analysis to examine transcriptional differences between CBs of both strains of mice. Experiment Overall Design: . This study utilizes microarray analysis to test these hypotheses. Three sets of CBs were harvested from both strains. RNA was isolated and used for global gene expression profiling (Affymetrix Mouse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05.
transcription profiling by array, unknown experiment type
A search for genes that may confer divergent morphology and function in the carotid body between two strains of mice. Alexander Balbir, Hannah Lee, Mariko Okumura, Shyam Biswal, Robert S Fitzgerald, Machiko Shirahata. Am J Physiol Lung Cell Mol Physiol 292(3):L704-15 (2007)