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E-GEOD-52883 - Comparison of gene expression profile of muscle samples stored under vacuum at +4°C up to 14 days from growth promoters-treated and control cows

Status
Released on 4 May 2015, last updated on 9 May 2015
Organism
Bos taurus
Samples (20)
Array (1)
Protocols (6)
Description
A bovine oligo microarray platform (GPL7053) was used to evidence differences in gene expression profiles of muscle samples from five (5) dexamethazone plus estradiol treated cows and from five (5) control animals. Samples were stored under vacuum at +4°C and collected at D0 and D14. In this study, we analyzed twenty (20) bovine neck muscle samples stored under vacuum at 4°C and collected at D0 (10) and D14 (10) from five (5) dexamethazone plus estradiol treated cows and five (5) from control animals. Gene expression profiling was performed using the Agilent-015354 Bos taurus Oligo Microarray platform (GPL7053) based on single-colour detection (Cyanine-3 only). Microarrays were scanned with Agilent scanner G2565BA (barcode on the left, DNA on the back surface, scanned through the glass) at a resolution of 5 microns; all slides were scanned twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%); the scanner software created a unique ID for each pair of XDR scans and saved it to both scan image files. Feature Extraction (FE) 9.5 used XDR ID to link the pairs of scans together automatically when extracting data. The signal left after all the FE processing steps have been completed is ProcessedSignal that contains the Multiplicatively Detrended, Background-Subtracted Signal.
Experiment type
transcription profiling by array 
Contacts
sara pegolo <sara.pegolo@unipd.it>, Luca Bargelloni, Sara Pegolo
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52883.idf.txt
Sample and data relationshipE-GEOD-52883.sdrf.txt
Raw data (1)E-GEOD-52883.raw.1.zip
Processed data (1)E-GEOD-52883.processed.1.zip
Array designA-GEOD-7053.adf.txt
Links