E-GEOD-52839 - Transcription factor Achaete-Scute homologue 2 initiates T follicular helper cell development [ChIP-Seq]

Released on 1 January 2014, last updated on 31 May 2014
Mus musculus
Samples (6)
Protocols (7)
In immune responses, activated T cells migrate to B cell follicles and develop to T follicular helper (Tfh) cells, a new subset of CD4+ T cells specialized in providing help to B lymphocytes in the induction of germinal centers 1-3. Although Bcl6 has been shown to be essential in Tfh cell function, it may not regulate the initial migration of T cells 4 or the induction of Tfh program as exampled by CXCR5 upregulation 5. Here, we show that the Achaete-Scute homologue 2 (Ascl2) gene that encodes a basic helix-loop-helix (bHLH) transcription factor 6, is selectively upregulated in its expression in Tfh cells. Ectopic expression of Ascl2 uniquely upregulates CXCR5 but not Bcl6 and downregulates CCR7 expression in T cells in vitro and accelerates T cell migration to the follicles and Tfh cell development in vivo. Combined transcriptome profiling and genome-wide occupancy analysis indicate that Ascl2 directly regulates Tfh-related genes while inhibits expression of Th1 and Th17 genes. Acute deletion of Ascl2 as well as blockade of its function with the Id3 protein in peripheral CD4+ T cells results in a failure in Tfh cell development and the germinal center response. Conversely, mutation of Id3, known to cause antibody-mediated autoimmunity, greatly enhances Tfh cell generation. Thus, Ascl2 critically and directly initiates Tfh cell development. Decide Ascl2 binding sites in CD4+ T cells
Experiment type
Lai Wei <weil2@mail.nih.gov>, Chen Dong, Xiaowei Yan, Xindong Liu
Transcription factor achaete-scute homologue 2 initiates follicular T-helper-cell development. Liu X, Chen X, Zhong B, Wang A, Wang X, Chu F, Nurieva RI, Yan X, Chen P, van der Flier LG, Nakatsukasa H, Neelapu SS, Chen W, Clevers H, Tian Q, Qi H, Wei L, Dong C. , Europe PMC 24463518
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-52839.idf.txt
Sample and data relationshipE-GEOD-52839.sdrf.txt
Processed data (1)E-GEOD-52839.processed.1.zip