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E-GEOD-52772 - ChIP-Seq analysis of PU.1, BATF, and JunB in murine BMDCs

Status
Released on 22 December 2013, last updated on 15 April 2014
Organism
Mus musculus
Samples (3)
Protocols (3)
Description
Purpose: The purpose of this study is to identify the genome-wide binding sites for IRF4 interaction partners PU.1, BATF, and JunB in dendritic cells. These ChIP-seq data were integrated with gene expression analysis in IRF4-sufficient and -deficient BMDCs in order to assemble an IRF4 gene regulatory network. Hematopoietic bone marrow progenitors from C57BL/6 mice were differentiated with GM-CSF and IL-4 for 5 days. On day 6, BMDCs were stimulated for 6 hours with 100ng/ml LPS. Fixed chromatin was immunoprecipitated with anti-PU.1, BATF, and JunB antibodies and subjected to high-throughput sequencing. The sequencing data for the input DNA was previously submitted as GSM999807.
Experiment type
ChIP-seq 
Contacts
Aly A Khan, Bryan Vander Lugt, Cecile Chalouni, Chauncey J Spooner, Harinder Singh, Ira Mellman, Jason A Hackney, Jason DeVoss, Justin Lesch, Lelia Delamarre, Meijuan Zhou, Min Xu, Smita Agrawal, Summer Park, Wyne P Lee, Zora Modrusan
Citation
Transcriptional programming of dendritic cells for enhanced MHC class II antigen presentation. Vander Lugt B, Khan AA, Hackney JA, Agrawal S, Lesch J, Zhou M, Lee WP, Park S, Xu M, DeVoss J, Spooner CJ, Chalouni C, Delamarre L, Mellman I, Singh H. , PMID:24362890
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-52772.idf.txt
Sample and data relationshipE-GEOD-52772.sdrf.txt
Processed data (1)E-GEOD-52772.processed.1.zip
Links