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E-GEOD-52746 - Expression data from intestinal mucosa of patients with CD under anti-TNF-alpha therapy

Status
Released on 8 April 2014, last updated on 12 April 2014
Organism
Homo sapiens
Samples (39)
Array (1)
Protocols (5)
Description
Crohn's Disease (CD) is a chronic inflammatory disease of the intestinal tract. We performed a whole-genome transcriptional analysis using colonic biopsies from CD patients before and after anti-TNF-α therapy. Involved colonic samples from Crohn's disease patients and healthy colonic samples from non-inflammatory controls were collected for RNA extraction and hybridization on Affymetrix microarrays. Inclusion criteria for CD patients were: age between 18 and 70, diagnosis of CD established at least 4 months before inclusion and exclusion of concomitant infection. Active disease was defined by endoscopic and clinical score: endoscopic active disease was defined as a CD endoscopic index of severity (CDEIS) of 5 or more and the presence of large ulcers (> 0.5 cm diameter) in at least one of the explored segments. Clinical activity was defined as a CD activity index (CDAI) above 150. Finally, a total of 39 biopsies were analyzed: 17 healthy controls, 10 active CD without anti-TNF therapy, 5 active CD with anti-TNF therapy (non-responders) and 7 inactive CD with anti-TNF therapy (responders).
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52746.idf.txt
Sample and data relationshipE-GEOD-52746.sdrf.txt
Raw data (1)E-GEOD-52746.raw.1.zip
Processed data (1)E-GEOD-52746.processed.1.zip
Array designA-GEOD-17996.adf.txt
Links