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E-GEOD-52701 - Transgenic yeast (pAG305GPD-AAE13) vs. control yeast (pAG305GPD-ccdB)

Status
Released on 26 November 2013, last updated on 2 May 2014
Organism
Saccharomyces cerevisiae
Samples (8)
Array (1)
Protocols (8)
Description
The yeast expression vectors used to express AAE13 was constructed as follows: AAE13 (At3g16170) was amplified from the vector pENTR-AAE13. The amplified product was cloned into pCR®8⁄GW⁄TOPO® (Invitrogen). The resulting plasmid was combined with the Advanced Gateway destination vectors pAG305GPD-ccdB (Addgene, Boston, MA) in an attL X attR recombination reaction to generate the vectors pAG305GPD-AAE13. The integrating vector pAG305GPD-AAE13 was introduced into WAT11, which was also transformed empty vector pAG305GPD-ccdB as control (B1, B2, B3 and B4). The transgenic and control yeast cells were cultured in SD-drop out medium at 30 °C under shaken conditions, malonate was fed into cells at final concentration 0.2 mM when the value of OD600 reached at 0.1. The cells were harvested at mid-log phase. Two-condition experiment, Transgenic yeast (pAG305GPD-AAE13) vs. control yeast (pAG305GPD-ccdB). Biological replicates: 4 control replicates, 4 transgenic replicates.
Experiment type
transcription profiling by array 
Contacts
Jinsheng Yu <jyu@wustl.edu>, Oliver Yu, Yechun Wang
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52701.idf.txt
Sample and data relationshipE-GEOD-52701.sdrf.txt
Raw data (1)E-GEOD-52701.raw.1.zip
Processed data (1)E-GEOD-52701.processed.1.zip
Array designA-GEOD-16244.adf.txt
Links