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E-GEOD-52640 - Transcript profiling of transgenic rice lines where the OsMADS26 gene is over-expressed or down growing cultivated in standard or osmotic stress condition

Status
Released on 20 November 2015, last updated on 29 November 2015
Organism
Oryza sativa
Samples (24)
Array (1)
Protocols (7)
Description
Functional analyses of MADS-box transcription factors in plants have unraveled their role in major developmental programs (e.g; flowering and floral organ identity), in stress-related developmental processes such as abscission, fruit ripening and senescence and the role of some of them in stress response regulation was reported. The aim of this study was to decipher the genes that are under the control of the OsMADS26 transcription factor in rice in standard or osmotic stress condition. Transcriptome analysis in over-expressor and down-regulated OsMADS26 rice transgenic lines revealed that several major regulators of biotic and abiotic stress responses are mis-regulated compared to control plants, suggesting that OsMADS26 acts as an upstream negative regulator of stress-associated genes. Transgenic plants over-expressing or down-regulated (by RNA interference) for OsMADS26 were obtained and used to study the impact of this transcription factor on gene regulation in plants cultivated in standard or osmotic stress (100 mM manitol) condition.
Experiment type
transcription profiling by array 
Contacts
Pascal Gantet <pascal.gantet@univ-montp2.fr>, Emmanuel Guiderdoni, Giang N Khong, Jean-Benoit Morel
Citation
OsMADS26 negatively regulates resistance to pathogens and drought tolerance in rice. Khong GN, Pati PK, Richaud F, Parizot B, Bidzinski P, Mai CD, B�s M, Bourri� I, Meynard D, Beeckman T, Selvaraj MG, Manabu I, Genga A, Brugidou C, Do VN, Guiderdoni E, Morel JB, Gantet P. , PMID:26424158
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52640.idf.txt
Sample and data relationshipE-GEOD-52640.sdrf.txt
Raw data (1)E-GEOD-52640.raw.1.zip
Processed data (1)E-GEOD-52640.processed.1.zip
Array designA-AFFY-126.adf.txt
Links