E-GEOD-5255 - Transcription profiling of whole lungs from mice expressing an inducible dominant negative BMPR2 transgene to study the effect of BMPR2 on gene expression
Submitted on 6 July 2006, released on 27 November 2007, last updated on 27 March 2012
Mice expressing a doxycycline-inducible dominant negative BMPR2 transgene expressed only in smooth muscle are activated for one or eight weeks, and compared to transactivator-only mice also fed doxycycline. All mice are 12 weeks of old at sacrifice. Experiment Overall Design: Each chip is a pool of three individual mice, to reduce variability. The transactivator-only mice are controls. The other mice all had elevated RV pressures at time of sacrifice. RNA was prepared from whole lung.
transcription profiling by array, co-expression, individual genetic characteristics, sex, time series
Molecular effects of loss of BMPR2 signaling in smooth muscle in a transgenic mouse model of PAH. Tada Y, Majka S, Carr M, Harral J, Crona D, Kuriyama T, West J. Am J Physiol Lung Cell Mol Physiol 292(6):L1556-63 (2007)