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E-GEOD-52539 - Global analysis of the nuclear processing of unspliced U12-type introns by the exosome

Status
Released on 26 May 2014, last updated on 20 June 2014
Organism
Homo sapiens
Samples (6)
Protocols (4)
Description
we analyzed globally the effect of exosome processing on the nuclear pre-mRNA transcripts by inactivating either the RRP41 or DIS3 subunit of the exosome. Using SOLiD RNA sequencing technology, we report 30-120 million mapped cellular compartment specific reads per sample allowing the detection of unspliced pre-mRNAs. We show that RRP41 and DIS3 knockdowns stabilize an overlapping set of U12-type introns. Studying the global effect of the exosome (Rrp41 or Dis3 subunit) knockdown comparing to the control sample.
Experiment types
other, RNA-seq of coding RNA, RNA-seq of non coding RNA 
Contacts
Mikko Juhani Frilander <geo@ncbi.nlm.nih.gov>, Ali Oghabian, Dario Greco, Elina H Niemelä, Ger Pruijn, Mikko J Frilander, Raymond H Staals
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-52539.idf.txt
Sample and data relationshipE-GEOD-52539.sdrf.txt
Additional data (1)E-GEOD-52539.additional.1.zip
Links