9 protocols
AccessionType
normalization data transformation protocol
The raw signal was quantile-normalized across samples in R. ID_REF = VALUE = quantile-normalized signal idelta-3.Detection Pval =
array scanning protocol
Scan protocol for Illumina MouseRef-8 v2.0 expression beadchip
normalization data transformation protocol
The raw signal was quantile-normalized across samples in R. ID_REF = VALUE = quantile-normalized signal idelta-2.Detection Pval =
normalization data transformation protocol
The raw signal was quantile-normalized across samples in R. ID_REF = VALUE = quantile-normalized signal idelta-1.Detection Pval =
hybridization protocol
Hyb protocol for Illumina MouseRef-8 v2.0 expression beadchip
labelling protocol
label protocol for Illumina MouseRef-8 v2.0 expression beadchip
sample treatment protocol
Pancreata were perfused through the common bile duct. After removing endogenous islets, the acinar fraction was digested with liberase and Elastase. Roche) into single cells.
growth protocol
Rag1-/- were obtained from Jackson Labs. For delta- cell induction, 100ul (>1x 109 pfu) of purified adenovirus was directly injected into the splenic lobe of the dorsal pancreas.
nucleic acid extraction protocol
Cherry+ induced beta cells were isolated by fluorescent activated cell sorting (FACS) with FACSaria (BD Bioscience), RNA samples were extracted by Trizol