normalization data transformation protocol
The data were normalised using quantile normalisation with IlluminaGUI in R ID_REF = VALUE = quantile normalized Detection Pval =
array scanning protocol
Standard Illumina scanning protocol
Standard Illumina hybridization protocol
Illumina Gene Expression protocols feature a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides.
nucleic acid extraction protocol
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.