E-GEOD-5229 - Transcription profiling of human and mouse keratinocytes infected with AD and Ad-NotchIC viruses reveals cross-regulation between Notch and p63 in keratinocyte commitment to differentiation
Submitted on 5 July 2006, released on 13 June 2008, last updated on 27 March 2012
Homo sapiens, Mus musculus
Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigenesis. p63, a p53 family member, has been implicated in establishment of the keratinocyte cell fate and/or maintenance of epithelial self-renewal. Here we show that p63 expression is suppressed by Notch1 activation in both mouse and human keratinocytes through a mechanism independent of cell cycle withdrawal and requiring down-modulation of selected interferon-responsive genes, including IRF7 and/or IRF3. In turn, elevated p63 expression counteracts the ability of Notch1 to restrict growth and promote differentiation. p63 functions as a selective modulator of Notch1-dependent transcription and function, with the Hes-1 gene as one of its direct negative targets. Thus, a complex cross-talk between Notch and p63 is involved in the balance between keratinocyte self-renewal and differentiation. Experiment Overall Design: PolyA+ mRNA (2-5 ug) from cells infected with the Ad GFP and Ad-NotchIC viruses was used as template for double stranded cDNA preparations with a T7-(dT)24 oligonucleotide primers for the first strand reaction. The resulting cDNA was used for preparation of biotin-labeled antisense cRNA and further used for hybridization to Affymetrix U74A or U95A “gene chips” according to the manufacturer’s recommendation.
transcription profiling by array, unknown experiment type
Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation. Bach-Cuc Nguyen, Karine Lefort, Anna Mandinova, Dario Antonini, Vikram Devgan, Giusy Della Gatta, Maranke I Koster, Zhuo Zhang, Jian Wang, Alice Tommasi di Vignano, Jan Kitajewski, Giovanna Chiorino, Dennis R Roop, Caterina Missero, G Paolo Dotto. Genes Dev 20(8):1028-42 (2006)