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E-GEOD-52141 - Comparison of gene expression profiles of miR-142-/- primary megakaryocytes and WT primary megakaryocytes

Status
Released on 11 June 2014, last updated on 13 June 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (6)
Description
Whole fetal livers were collected from mouse fetuses at embryonic day 14.5 (E14.5), and single-cell suspensions were prepared by successive passage through 18-, 21 and 23-gauge needles. Fetal liver cells were maintained in Dulbecco modified Eagle medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, 100µg/ml streptomycin, and 50ng/ml recombinant human thrombopoietin (TPO; Peprotech). After 5 days of culture, megakaryocytes were purified using a discontinuous bovine serum albumin gradient (BSA, Sigma–Aldrich; 3%, 1.5%, and 0%). Total RNA was isolated with Tri–Reagent (MRC) following manufacturer’s instructions, and its quality was assessed with ND–1000 Nanodrop (Peqlab) and on a 1.5% agarose gel. Gene expression was measured in primary megakaryocytes cultured from miR-142-/- fetal livers and wild type fetal livers at E14.5. Two independent experiments were performed.
Experiment type
transcription profiling by array 
Contacts
Citation
miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis. Chapnik E, Rivkin N, Mildner A, Beck G, Pasvolsky R, Metzl-Raz E, Birger Y, Amir G, Tirosh I, Porat Z, Israel LL, Lellouche E, Michaeli S, Lellouche JP, Izraeli S, Jung S, Hornstein E. , PMID:24859754
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52141.idf.txt
Sample and data relationshipE-GEOD-52141.sdrf.txt
Raw data (1)E-GEOD-52141.raw.1.zip
Processed data (1)E-GEOD-52141.processed.1.zip
Array designA-AFFY-130.adf.txt
Links