7 protocols
normalization data transformation protocol
The CEL Files were generated using the Affymetrix GeneChip Command Console (AGCC) software 3.0. Data were normalized using Affymtrix Expression Console software using MAS5 statistical algorithm. ID_REF = VALUE = MAS5.0 signal intensity ABS_CALL =
array scanning protocol
The arrays were read with the GeneChip scanner 3000 7G (Affymetrix).
hybridization protocol
Following an Affymetrix protocol, 10 ug of labeled cRNA were fragmented and hybridized on GeneChip Human Genome U133 plus 2.0 array (Affymetrix) during 16 hours at 45°C. Arrays were washed and stained with streptavidin-phycoerythrin (GeneChip Hybridization Wash and Stain kit) in the Affymetrix Fluidics Station 450.
labelling protocol
Total RNA was triple amplified using an ExpressArt mRNA amplification Pico kit (Amptec GmbH) and biotin-labelled by a round of in vitro transcription using a BioArray HighYield RNA transcript Labeling kit (Enzo Life Sciences) following he manufacturer's protocol.
nucleic acid extraction protocol
RNA extraction were performed using the Rneasy Micro kit (Qiagen).
sample treatment protocol
Spinal cord sections were dehydrated and microdissected using the PixCell LCM System (Arcturus)
growth protocol
Snap frozen spinal cord samples were used.