E-GEOD-52127 - Brain tumor initiating cell response to microglia

Status
Released on 6 November 2013, last updated on 18 June 2015
Organism
Homo sapiens
Samples (7)
Array (1)
Protocols (5)
Description
Determination of the mechanism by which microglia regulate growth of brain tumor initiating cells (BTICs) and differentiation. Results identify the factors involved in the regulation and provide mechanistic basis. We subjected brain tumor initiating cells to microarray to determine the genes involved in BTICs growth and differentiation when exposed to microglia conditioned medium (MCM) for 6h. BTICs were grown in MCM or control medium for 6h, and media was removed and then the cells were subjected for RNA extraction and hybridization on Affymatrix microarrays. Three (3) control and four (4) MCM samples were generated. The overall objective was to collect the RNA from the MCM exposed differentiated BTICs.
Experiment type
transcription profiling by array 
Contacts
Voon Wee Yong <vyong@ucalgary.ca>, Susobhan Sarkar, V W Yong
Citation
Therapeutic activation of macrophages and microglia to suppress brain tumor-initiating cells. Sarkar S, Döring A, Zemp FJ, Silva C, Lun X, Wang X, Kelly J, Hader W, Hamilton M, Mercier P, Dunn JF, Kinniburgh D, van Rooijen N, Robbins S, Forsyth P, Cairncross G, Weiss S, Yong VW.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52127.idf.txt
Sample and data relationshipE-GEOD-52127.sdrf.txt
Raw data (1)E-GEOD-52127.raw.1.zip
Processed data (1)E-GEOD-52127.processed.1.zip
Array designA-AFFY-44.adf.txt
Links