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E-GEOD-51996 - Cell-type specific transcriptome analysis to study germline development and reproduction in apomictic Boechera gunnisoniana- ATH1 arrays

Status
Released on 1 June 2014, last updated on 7 June 2014
Organism
Boechera gunnisoniana
Samples (7)
Array (1)
Protocols (7)
Description
Apomixis differs from sexual reproduction only in three major aspects: While the sexual megaspore mother cell undergoes meiosis, the apomictic initial cell omits or aborts meiosis (apomeiosis); the unreduced egg cell of apomicts forms an embryo without fertilization (parthenogenesis), and formation of functional endosperm requires specific developmental adaptations. Currently, our knowledge about the gene regulatory programs underlying apomixis is scarce. We used the apomict Boechera gunnisoniana, a close relative of Arabidopsis thaliana, to investigate the transcriptional basis underlying apomeiosis and parthenogenesis. Here, we present the first comprehensive reference transcriptome for reproductive development in an apomictic species. To compare sexual and apomictic development at the cellular level, we then used a combination of laser-assisted microdissection with microarray and RNA-Seq analysis. Our study yields important new insights into the transcriptional basis underlying apomixis. Cell-type specific transcriptome profiles were generated from the apomictic initial cell (2 biological replicates), surrounding sporophytic tissues (sporo_nucellus; 2 biological replicates), egg cell, central cell and synergid cells (one sample each) from the triploid pseudogamous obligate apomict Boechera gunnisoniana by heterologous hybridization on the Affymetrix ATH1 arrays.
Experiment type
transcription profiling by array 
Contacts
Marc William Schmid <Schmid.M@access.uzh.ch>, Anja Schmidt, Marc Schmid, Ueli Grossniklaus
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-51996.idf.txt
Sample and data relationshipE-GEOD-51996.sdrf.txt
Raw data (1)E-GEOD-51996.raw.1.zip
Processed data (1)E-GEOD-51996.processed.1.zip
Array designA-GEOD-16112.adf.txt
Links