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E-GEOD-5189 - Expression data from adult male and female Drosophila melanogaster antenna, proboscis, and front leg

Status
Released on 10 June 2010, last updated on 27 March 2012
Organism
Drosophila melanogaster
Samples (24)
Array (1)
Protocols (2)
Description
Sex-specific gene expression in sensory organs may play an important role in mating and foraging behavior. We used long-oligonucleotide microarrays to compare gene expression profiles of males and females in three adult appendages that carry large numbers of chemosensory organs – antenna, proboscis, and front leg. Keywords: tissue-specific expression profiles Drosophila isogenic line WI89 was used. Adult male and female antennae (a3+arista), front legs (from distal tibia down), and proboscises were dissected at 1-2 hours after eclosion. Total RNA was extracted and the mRNA fraction was amplified by reverse transcription followed by in vitro transcription with T7 RNA polymerase.
Experiment type
transcription profiling by array 
Contacts
Lauren M McIntyre <mcintyre@UFL.EDU>, Lauren M. McIntyre <mcintyre@ufl.edu>, Artyom Kopp, Lawrence Harshman, Marta L Wayne, Olga Barmina, Sergey V Nuzhdin
Citation
Simpler mode of inheritance of transcriptional variation in male Drosophila melanogaster. Wayne ML, Telonis-Scott M, Bono LM, Harshman L, Kopp A, Nuzhdin SV, McIntyre LM.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5189.idf.txt
Sample and data relationshipE-GEOD-5189.sdrf.txt
Raw data (1)E-GEOD-5189.raw.1.zip
Processed data (1)E-GEOD-5189.processed.1.zip
Array designA-GEOD-3884.adf.txt
Links